Chromatin assembly. Relationship of chromatin structure to DNA sequence during simian virus 40 replication.
نویسندگان
چکیده
منابع مشابه
Chromatin: Nucleosome assembly during DNA replication
In the cells of higher eukaryotes, the genomic DNA, which is of a few metres in length, is compacted in such a way as to be confined within a nucleus only micrometres in diameter. This impressive compaction is brought about by association of the DNA with nuclear proteins, of which histones are the most prevalent. The resulting complex nucleoprotein structure is known as chromatin, and the basic...
متن کاملRelationship of replication and transcription of Simian Virus 40 DNA.
RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was...
متن کاملInteraction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
We have studied the binding of the tumor antigen (T-antigen) of simian virus 40 to simian virus 40 chromatin (minichromosomes). The minichromosomes isolated from infected cells by a modification of standard techniques were relatively free of contaminating RNA and cellular DNA and had a ratio (by weight) of protein to DNA of approximately 1; their DNA was 50 to 60% digestible to an acid-soluble ...
متن کاملUnwinding of parental strands during simian virus 40 DNA replication.
Pools of young (less than 60% replicated) and mature (60-90% replicated) replicating molecules of simian virus 40 (SV40) DNA have been treated at pH 12.2 in order to dissociate growing chains from the parental strands. The molecules are neutralized so that the parental strands can reassociate and they have then been isolated. They are covalently closed structures which sediment rapidly in alkal...
متن کاملChromatin dynamics during DNA replication.
Chromatin is composed of DNA and histones, which provide a unified platform for regulating DNA-related processes, mostly through their post-translational modification. During DNA replication, histone arrangement is perturbed, first to allow progression of DNA polymerase and then during repackaging of the replicated DNA. To study how DNA replication influences the pattern of histone modification...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)68919-2